The selected facultative mixotrophic sulphur-oxidizing bacteria from intensive shrimp ponds.

Ardiansyah, Ardiansyah and Amrullah, Amrullah and Dahlia, Dahlia and Jaya, Andi Asdar and Indrayani, Indrayani and Wahidah, Wahidah and Hamal, Rimal and Jabbar, Firmansyah (2020) The selected facultative mixotrophic sulphur-oxidizing bacteria from intensive shrimp ponds. AACL Bioflux, 13 (5). pp. 2886-2896. ISSN 1844-9166 (online); ISSN 1844-8143 (print)

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Abstract

The research was conducted to characterize and screen sulphur-oxidizing bacteria (SOB) from three intensive shrimp farms in the province of South Sulawesi, Indonesia. Bacteria were isolated using sulphur-oxidation medium (SOM) with the direct plating method. A volume of 0.1 mL of the diluted sample was poured into SOM agar medium and incubated at 30°C for 24 hours. A total number of 48 facultative mixotrophic SOB isolates were obtained and considered as positive SOB due to their abilities using thiosulphate for growth. Three SOB isolates such as SOB15, SOB26, and SOB31 were selected based on their abilities to grow faster, produce the highest sulphate ion and reducing the pH in the growth medium. Results showed that the optimum pH of all SOB isolates occurred at pH 8.0. Meanwhile, the optimum temperature for SOB15, SOB26, and SOB31 isolates occurred at 35°C. The three isolates were classified as facultative mixotrophic with the capability of growth in thiosulphate medium supplemented with yeast extract. This research reveals the ability of the selected SOB in the oxidation of thiosulphate, temperature and pH adaptabilities, with the metabolic flexibilities of isolates SOB15, SOB26, and SOB31 can be the prospective H2S removal agent in intensive shrimp farms.

Item Type:	Article
Subjects:	FAKULTAS TEKNIK FAKULTAS TEKNIK > Pendidikan Teknologi Pertanian
Divisions:	FAKULTAS TEKNIK
Depositing User:	Dr. Indrayani Indrayani
Date Deposited:	02 May 2023 04:32
Last Modified:	02 May 2023 04:32
URI:	http://eprints.unm.ac.id/id/eprint/28065

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