Antibacterial activity of the epidermal mucus of Nile tilapia (Oreochromis niloticus) against pathogens in vannamei shrimp (Litopenaeus vannamei Boone).

Ardiansyah, Ardiansyah and Amrullah, Amrullah and Dahlia, Dahlia and Indrayani, Indrayani and Atmomarsono, Muhariyadi and Jabbar, Firmansyah (2021) Antibacterial activity of the epidermal mucus of Nile tilapia (Oreochromis niloticus) against pathogens in vannamei shrimp (Litopenaeus vannamei Boone). AACL Bioflux, 14 (6). pp. 3538-3546. ISSN 1844-9166 (online); ISSN 1844-8143 (print)

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Abstract

Shrimp production is affected by disease attacks caused by various types of pathogenic Vibrio species. Mucus secreted in the epidermis of the fish skin acts as the first line of defence between fish and pathogens in their living medium. The purpose of this study was to evaluate the antimicrobial potential of Nile tilapia (Oreochromis niloticus) epidermal mucus against pathogenic bacteria in white shrimp (Litopenaeus vannamei Boone). Crude, acid, and aqueous mucin extracts of the epidermis were prepared, and their antibacterial activity was tested by disc diffusion method against three bacterial pathogens of vannamei shrimp; Vibrio harveyi, V. parahaemolyticus, and V. alginolyticus. The antibacterial activity was measured in the zone of inhibition in mm and compared with the antibiotic Streptomycin as a positive control, distilled water, and acetic acid as negative controls. Of the 27 tests performed (three types of epidermal mucin extract against three different bacterial strains with three repetitions), 15 tests showed antibacterial activity. Acid extracts showed inhibitory potential against pathogenic bacterium V. harveyi, crude and acidic extracts were able to inhibit V. parahaemolyticus, while V. alginolyticus could be inhibited by the three types of epidermal mucin extracts. O. niloticus acid extract showed inhibitory activity for all types of test pathogens; V. harveyi, V. Parahaemolyticus, and V. alginolyticus. The acidic extract of O. niloticus significantly affected V. harveyi, V. parahaemolyticus, and V. alginolyticus at a minimum concentration of 20 μL mL-1, but the aqueous extract of O. niloticus required 60 μL mL-1 of protein to inhibit V. harveyi. O. niloticus epidermal mucin can be a natural product, which can help overcome the problem of antibiotic resistance of many pathogenic bacteria in shrimp culture.

Item Type:	Article
Subjects:	FAKULTAS TEKNIK FAKULTAS TEKNIK > Pendidikan Teknologi Pertanian
Divisions:	FAKULTAS TEKNIK
Depositing User:	Dr. Indrayani Indrayani
Date Deposited:	02 May 2023 04:31
Last Modified:	02 May 2023 04:31
URI:	http://eprints.unm.ac.id/id/eprint/27871

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