THE SPATIOTEMPORAL DYNAMICS OF NUCLEOPORIN NUP58 DURING CELL DIVISION

Hartono, Hartono and Hazawa, Masaharu and Dewi, Firli Rahmah Primula and Kobayashi, Akiko and Mohamed, Mahmoud Shaaban and Siang, Lim Kee and Wong, Richard W. (2018) THE SPATIOTEMPORAL DYNAMICS OF NUCLEOPORIN NUP58 DURING CELL DIVISION. In: The 41st Annual Meeting of the Molecular Biology Society of Japan, 28-30 November 2018, Yokohama, Japan. (Unpublished)

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Abstract

Nuclear pore complexes (NPCs) are transport channels between the nucleus and the cytoplasm. The NPCs are composed by around 30 Nuclear pore complexes (NPCs) are transport channels between the nucleus and the cytoplasm. The NPCs are composed by around 30 different proteins, termed nucleoporins (Nups) and each Nup is present in multiple copies. Recently, we and others discovered that several nucleoporins play critical roles during cell division including centrosome integrity, kinetochore assembly, spindle formation and orientation. Nup58 is a part of the central transport channel of the NPC, which forms a complex protein with other nucleoporins such as Nup62 and Nup54. Where Nup58 localize and what their roles during cell division are still poorly understood. Here, we show that Nup58 localizes at the Nuclear pore complexes (NPCs) are transport channels between the nucleus and the cytoplasm. The NPCs are composed by around 30 different proteins, termed nucleoporins (Nups) and each Nup is present in multiple copies. Recently, we and others discovered that several nucleoporins play critical roles during cell division including centrosome integrity, kinetochore assembly, spindle formation and orientation. Nup58 is a part of the central transport channel of the NPC, which forms a complex protein with other nucleoporins such as Nup62 and Nup54. Where Nup58 localize and what their roles during cell division are still poorly understood. Here, we show that Nup58 localizes at the centrosome during prophase to metaphase, interacting with Nup62, Nup54 and also two centrosome marker protein gamma tubulin and SAS6. Depletion of Nup58 leads to monopolar spindle formation, multi-nucleic cell formation, and delayed cytokinesis. We suggest that Nup58 may regulate cytokinesis abscission. Our study gives novel insight into the role of Nup58 in cell division. centrosome during prophase to metaphase, interacting with Nup62, Nup54 and also two centrosome marker protein gamma tubulin and SAS6. Depletion of Nup58 leads to monopolar spindle formation, multi-nucleic cell formation, and delayed cytokinesis. We suggest that Nup58 may regulate cytokinesis abscission. Our study gives novel insight into the role of Nup58 in cell division. different proteins, termed nucleoporins (Nups) and each Nup is present in multiple copies. Recently, we and others discovered that several nucleoporins play critical roles during cell division including centrosome integrity, kinetochore assembly, spindle formation and orientation. Nup58 is a part of the central transport channel of the NPC, which forms a complex protein with other nucleoporins such as Nup62 and Nup54. Where Nup58 localize and what their roles during cell division are still poorly understood. Here, we show that Nup58 localizes at the centrosome during prophase to metaphase, interacting with Nup62, Nup54 and also two centrosome marker protein gamma tubulin and SAS6. Depletion of Nup58 leads to monopolar spindle formation, multi-nucleic cell formation, and delayed cytokinesis. We suggest that Nup58 may regulate cytokinesis abscission. Our study gives novel insight into the role of Nup58 in cell division.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	FMIPA
Divisions:	FAKULTAS MIPA
Depositing User:	Zainatun
Date Deposited:	30 Jun 2023 04:13
Last Modified:	30 Jun 2023 04:13
URI:	http://eprints.unm.ac.id/id/eprint/32400

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