Development of Chitosan Based siRNA-PLK1 Nanoparticles as Anticancer

Hartono, Hartono and Hasri, Hasri and Yustisia, Ika and Ladj, Rusdina Bte and Rapi, Irma and Risdayanti, Eka and Afdalia, St.Sufia and Amelia, Tiara Putri and Basri, Nur Putri Apriliani Development of Chitosan Based siRNA-PLK1 Nanoparticles as Anticancer. In: The 2nd International Conference on Life Science and Biology Education, 15 November 2022, Makassar, Indonesia. (Unpublished)

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Abstract

PLK1 is one of the kinase enzymes that has a very important role in controlling eukaryotic cell cycle. Inhibition of PLK1 gene expression with small interfering RNA (siRNA) molecule impede cancer cell division leading to cell death. Therefore, PLK1 is recommended as a potential target gene for cancer therapy. The development of chitosan-based siRNA-PLK1 nanoparticles is expected to be a safe and effective alternative for cancer therapy. The purpose of this study was to develop a chitosan-based siRNAPLK1 nanoparticle product as an anti-cancer. The chitosan-based siRNA-PLK1 nanoparticles ws developed using the ionic gelation method. Nanoparticle characterization and encapsulation efficiency of siRNA-PLK1 nanoparticles were measured by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) and Ultraviolet-visible (UV-Vis) spectroscopy analysis. The results showed that conjugation of chitosan with siRNA-PLK1 through the ionic gelation method has succeeded in forming a stable nanoparticle structure.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	FMIPA
Depositing User:	Zainatun
Date Deposited:	26 Jun 2023 06:47
Last Modified:	26 Jun 2023 06:47
URI:	http://eprints.unm.ac.id/id/eprint/31402

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