Arsita, Rajidah and Karim, Hilda and Hala, Yusminah and Iriany, R. Neni and Jumadi, Oslan (2020) Isolation and identification of nitrogen-fixing bacteria in the corn rhizosphere (Zea mays l.) originating from Jeneponto Regency, South Sulawesi. IOP Conference Series: Earth and Environmental Science, 484 (012051). pp. 1-7. ISSN 1755-1315
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Abstract
Abstract This study aims to determine the type of isolates and the characteristics of nitrogen-fixing bacteria in the rhizosphere of corn (Zea mays L.) from Jeneponto regency, South Sulawesi. This research is an explorative study consisting of nitrogen-fixing bacteria isolation and identification using nitrogen free bromohymol blue (Nfb) medium, and an ammonium excretion test on nitrogen-fixing bacteria using spectrophotometry methods. Bacteria identification methods include Gram staining, and physiological and biochemical characterization which consist of temperature resistance at 5°C, 25°C, and 40°C, pH resistance at 4, 6, and 8 and salt tolerance at 1%, 6% and 10 %. The study has found 20 bacterial isolates that can be characterized as round, mucoid, non-mucoid, white, clear, Gram-negative and bacilli. Physiological characteristics (biochemical tests) have shown growing pellicles and discoloration in the liquid Nfb medium. The ammonium excretion test showed there are 5 isolates excreting fairly high ammonium levels, namely BKr1, BKr2, BKr3, TKN2 and BBTg1, with concentrations of 42.3, 39.1, 41.5, 41.0 and 39.4 μत, respectively.
Item Type: | Article |
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Subjects: | KARYA ILMIAH DOSEN Universitas Negeri Makassar > KARYA ILMIAH DOSEN |
Divisions: | KOLEKSI KARYA ILMIAH UPT PERPUSTAKAAN UNM MENURUT FAKULTAS > KARYA ILMIAH DOSEN KARYA ILMIAH DOSEN |
Depositing User: | Herling HR Sahade |
Date Deposited: | 21 Jul 2022 15:41 |
Last Modified: | 21 Jul 2022 15:41 |
URI: | http://eprints.unm.ac.id/id/eprint/24066 |
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