Isolation and Optimization of Endophytic Bacteria from Roots of Karst Area Ecosystems Producing Protease Enzymes

Pagarra, Halifah and Hartati, Hartati and Ali, Alimuddin and Salempa, Pince and Muharram, Muharram (2020) Isolation and Optimization of Endophytic Bacteria from Roots of Karst Area Ecosystems Producing Protease Enzymes. The Journal of Research on the Lepidoptera, 51 (2). pp. 431-439. ISSN ISSN 0022-4324 (print) ISSN 2156-5457 (online)

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Abstract

This study aims to determine the activity of the protease enzyme from bacterial A isolates isolated from Karst plant roots in various incubation time variations. Bacterial isolates used were isolates of bacteria A, proteolytic thermophilic bacteria isolated from Karst Ecosystem in Pangkajene Regency, South Sulawesi Province. Testing of protease activity was carried out using the Lowry method. The highest crude protein content of all treatments was obtained at 48 hours incubation time for Bacterial Isolates A (8.329 mg / ml). The results showed that Bacterial Isolate A showed an optimal production time at 48 hours with an enzyme activity of 0.423 Units/ml. While the exponential phase of bacterial growth on 48-hour incubation act with a maximum optical density of 1.444. It can be concluded that Bacterial Isolate A is a proteolytic bacterial characterized by the formation of a clear zone around the bacterial colony on the SMA. The production of protease enzymes obtained through RSM (CCD) method is optimum at 0.485 U / ml at glucose concentration of 0.60%, yeast extract 1.50% and skim milk 3.00%. SDS-PAGE analysis showed that the protease enzyme from Isolate A was 15, 20 and 37 kDa.

Item Type:	Article
Subjects:	FMIPA > BIOLOGI - (S1) FMIPA KARYA ILMIAH DOSEN Universitas Negeri Makassar > KARYA ILMIAH DOSEN
Divisions:	KOLEKSI KARYA ILMIAH UPT PERPUSTAKAAN UNM MENURUT FAKULTAS > KARYA ILMIAH DOSEN KARYA ILMIAH DOSEN
Depositing User:	Ir., Ph.D Halifah Pagarra
Date Deposited:	27 May 2020 23:35
Last Modified:	26 Jan 2021 09:31
URI:	http://eprints.unm.ac.id/id/eprint/17668

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