Ali, Alimuddin (2018) Screening of Endophytic Bacteria Producing Antifungal isolated from Indonesia Medicinal Plant, Java Ginseng (Talinum triangulare)(Jacq.) Willd. International Journal of Pharmacy and pharmaceutical sciences. ISSN 0975-1491
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Abstract
The objective of this research was to isolate and characterize endophytic bacteria from Talinum triangulare having antifungal activity.The endophytic bacteria were isolated from roots tissue of Talinum triangulare by surface sterilization method. The isolates werecultured in Trypticase Soybean agar and antagonist activities were evaluated by dual culture assay against Fusarium oxysporum, Trichoderma reeseiand Candida albicans. For metabolite antifungal activities, bacterial isolates were grown for 4 d in TS broth at 35 °C under shaking condition. Theantifungal activities of the supernatant extract were determined by using the disk agar diffusion. Polyketide synthase (PKS I) and NRPS genesfragments of all isolates were amplified.The result reveals that 4 of 23 endophytic bacterial isolates demonstrated great antifungal potentiality against many tested fungi.Polyketide synthase (PKS I) and NRPS genes amplification were showed 10 and 4 of endophytic isolates detected harboring PKS type I and NRPSgenes, respectively. In general, high frequencies of positive PCR amplification were obtained for PKS I (43.47%). Phylogenetic analyses based on the16S rRNA gene sequence, morphological, physiological and biochemical showed that the isolates were identified as a member of genus Bacillus andBrevibacillus. Keywords: Endophytic bacteria, Antifungal, Talinum triangulare, PKS I, NRPS, 16S rRNA
| Item Type: | Article |
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| Subjects: | KARYA ILMIAH DOSEN Universitas Negeri Makassar > KARYA ILMIAH DOSEN |
| Divisions: | KOLEKSI KARYA ILMIAH UPT PERPUSTAKAAN UNM MENURUT FAKULTAS > KARYA ILMIAH DOSEN KARYA ILMIAH DOSEN |
| Depositing User: | UPT PERPUSTAKAAN UNM |
| Date Deposited: | 10 Sep 2019 07:26 |
| Last Modified: | 10 Sep 2019 07:26 |
| URI: | http://eprints.unm.ac.id/id/eprint/14851 |
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