Pagarra, Halifah and Rahman, Roshanida A and Rachmawaty, Rachmawaty (2019) Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation. EurAsian Journal of BioSciences, 13. pp. 199-206. ISSN 13079867
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Abstract
The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment.
| Item Type: | Article |
|---|---|
| Subjects: | FMIPA > BIOLOGI - (S1) FMIPA KARYA ILMIAH DOSEN Universitas Negeri Makassar > KARYA ILMIAH DOSEN |
| Divisions: | FAKULTAS MIPA |
| Depositing User: | Ir., Ph.D Halifah Pagarra |
| Date Deposited: | 12 Aug 2019 04:06 |
| Last Modified: | 12 Aug 2019 04:06 |
| URI: | http://eprints.unm.ac.id/id/eprint/14495 |
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