OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION

Pagarra, Halifah and Rahman, Roshanida A and Azelee, Nur Izyan Wan and Illias, Rosli Md (2019) OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION. Jurnal Teknologi, 81 (1). pp. 59-67. ISSN 2180-3722

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Official URL: https://jurnalteknologi.utm.my/index.php/jurnaltek...

Abstract Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology. Keywords: Nephrolepis biserrata, Exo-polygalacturonase, Solid-state fermentation, Response Surface Methodology and Aspergillus niger

Item Type: Article
Subjects: FMIPA > BIOLOGI - (S1)
FMIPA
KARYA ILMIAH DOSEN
Universitas Negeri Makassar > KARYA ILMIAH DOSEN
Divisions: FAKULTAS MIPA
Depositing User: Ir., Ph.D Halifah Pagarra
Date Deposited: 08 Jan 2019 08:39
Last Modified: 12 Aug 2019 22:28
URI: http://eprints.unm.ac.id/id/eprint/11735

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