Sukainah, Andi and Johannes, Eva and Praja Putra, Reski (2017) Isolasi dan Identifikasi Bakteri Indigenus pada Tepung Jagung Bisi 16 selama Proses Fermentasi Spontan. Prosiding Seminar Nasional PATPI 2017 "Peran Ahli Teknologi pangan dalam mewujudkan ketahanan pangan nasional". p. 1176. ISSN 9766027200630
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Abstract
Andi Sukainah1)*, Eva Johannes 2), Reski Praja Putra 1) Program Studi Pendidikan Teknologi Pertanian, Fakultas Teknik, Universitas Negeri Makassar, Makassar, Indonesia Jurusan Biologi, Fakultas MIPA, Universitas Hasanuddin, Makassar, Indonesia Indigenous microbes are a group of microbes that are involved and discovered during the spontaneous fermentation process of Corn flour Bisi 16. One type of bacteria involved and is found during the spontaneous fermentation process of corn flour Bisi 16 is lactic acid bacteria (LAB). However, apart from LAB, during the spontaneous fermentation process of corn flour Bisi 16 was also found another type of bacteria. The purpose of this study was to isolate and identify indigenous bacteria other than BAL involved in the spontaneous fermentation process of Corn flour Bisi 16. Isolate obtained from the isolation stage will be identified its morphological properties. The observed morphological properties are cell shape, Gram staining, catalase test, and endospora staining. Subsequently, pure isolates will be analyzed their genotypes using Polymerase Chain Reaction (PCR) method and 16S rRNA sequencing analysis. The results showed that ASN9 and ASN11 isolates obtained during the spontaneous fermentation process of corn meal had a white and round coloni shape. The ASN9 isolate on the edges of the colony is rather gummy. Both isolates have rod-shaped cells, Gram (-), catalase (+), and endospores (-). Results of the 16S rRNA sequencing analysis, both ASN9 and ASN11 isolates, showed both isolates were genotypically similar to the Enterobacter cloacae subsp. cloacae with a similarity rate of 98% (ASN9 isolates) and ASN11 isolates, ie 99%. Keywords : corn flour, spontaneous fermentation, isolation, identification, Enterobacter cloacae subsp. cloacae
| Item Type: | Article |
|---|---|
| Subjects: | KARYA ILMIAH DOSEN Universitas Negeri Makassar > KARYA ILMIAH DOSEN |
| Divisions: | FAKULTAS ILMU PENDIDIKAN FAKULTAS ILMU PENDIDIKAN |
| Depositing User: | UPT PERPUSTAKAAN UNM |
| Date Deposited: | 25 Sep 2018 05:08 |
| Last Modified: | 25 Sep 2018 05:08 |
| URI: | http://eprints.unm.ac.id/id/eprint/10938 |
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